If something is rotthing in your kitchen right now,use it and make out of it life-saving antibiotics!Penicillin has been around a lot longer than people think. In fact the American Indians used it centuries before the white man ever ventured onto America’s shores. Penicillin is made by the blue and white penicillium molds.Making penicillin at home is difficult, but possible if you have the right equipment and ingredients.
You will need:
A gram scale
A 1-liter glass container
750 ml Erlenmeyer flask with a non-absorbent plug
A pH test kit
2 pieces of whole wheat bread
A cantaloupe rind, more bread, or citrus fruit
– Set out the rind, bread or fruit and let it mold . It will go through a few phases. First the mold will be white or gray, then it will turn blue, then a bright blue-green. This is the color you want. Note: if you choose to use bread, it’s best to make it yourself because many bakeries use an ingredient that inhibits mold growth.
–Sterilize the flask by putting it in the pressure cooker at 15 lb. for at least 15 minutes, or bake it at 315 degrees F for an hour.
–Cut the whole wheat bread (see note in step 1) into 1/2-inch cubes and place them in the flask, careful to be as sterile as you can.
–Scrape the blue-green mold from the host and place it in with the bread. Again, be as sterile with this step as you can, for instance, boil the tongs that you’re using.
–Place the flask in a dark place that’s around 70 degrees and allow it to incubate for 5 days.
–Now it’s going to get complicated. You’re going to need the following ingredients:
Lactose Monohydrate 44 gm
Corn Starch 25 gm
Sodium Nitrate 3 gm
Magnesium Sulfate 0.25 gm
Potassium Monophosphate 0.50 gm
Glucose Monohydrate 2.75 gm
Zinc Sulfate 0.044 gm
Manganese Sulfate 0.044 gm
–Sterilize the 1+ liter container, then dissolve the above ingredients in 500 ml of cold water. Add more cold water to make it a full liter.
–Use hydrochloric acid (HCL) to adjust the pH to 5.0-5.5 using your pH test kit.
–Sterilize the container along with the solution as described above.
–Allow the solution to cool, then add the mold. Incubate it for another 7 days under the same conditions as before.
Now it’s time to extract the penicillin that’s infused in the fluid.
– Filter the mix through a coffee filter or sterilized cheesecloth.
–Adjust the pH of the solution to 2.2 using the HCL and the pH test kit.
–Mix with cold ethyl acetate in the separatory funnel and shake vigorously for 30 seconds or so then allow it to separate. The ethyl acetate will be at the bottom.
–Chill a beaker in an ice bath and drain the ethyl acetate into it. Add 1 percent potassium acetate and mix it again.
–Let the ethyl acetate evaporate off while the solution is still in the beaker. You want plenty of air circulating through.
–You have penicillin, assuming you did everything right. Actually the crystals that remain are potassium penicillin and potassium acetate.
Safest way for using it is by making a poultice or patch and applying it to the afflicted area. But if the infection is internal, you’ll need to dry the stuff up, grind it to a powder, then feed it to whoever is sick.
Penicillin works against gram-positive bacteria, such as Staphylococcus and Pneumococcus by disrupting bacterial cell wall synthesis causing the cells to take on excess water, which causes them to burst. Effectiveness against gram-negative bacteria (such as E. coli and K. pneumoniae) is limited, with very high concentrations of penicillin needed to kill those organisms, which have membranes that protect them from penicillin.
Some gram-positive bacteria have developed the ability to survive penicillin exposure (become resistant to it) by producing penicillinases, enzymes that degrade penicillin.
Penicillins paved the way for other antibiotics, such as cephalosporins, made from the fungus Acremonium, and streptomycin from Actinomycetes Streptomyces.